Defender Ben Davies and midfielder Dylan Levitt will miss Wales’ friendly against the Czech Republic on Tuesday.
Davies has returned to Tottenham as a precaution, while Levitt – on loan at Dundee United from Manchester United – has left Wales’ squad due to illness.
Davies played the full 90 minutes of Thursday’s 2-1 win over Austria in their World Cup play-off semi-final.
“He’s played a lot of football… there’s no point risking him,” said Wales manager Robert Page.
The FAW has already announced funds from the game against the Czechs will go towards the Disasters Emergency Committee (DEC) Ukraine Humanitarian Appeal.
Because of Russia’s invasion, Ukraine’s semi-final play-off against Scotland has been postponed until June.
Wales will meet the winner of that encounter, with a place at the 2022 Qatar World Cup at stake.
